KMID : 1098419970050020102
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Korean Journal of Medicinal Crop Science 1997 Volume.5 No. 2 p.102 ~ p.107
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Effect of Ethylene Inhibitors on Plant Regeneration of Angelica keiskei Koidz
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Lee Joong-Ho
Gwon Tae-Oh Namkoong Seung-Bak Park Byung-Woo
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Abstract
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This study was carried out to increase the rate of plant regeneration from embryogenic callus of A. keiskei on MS medium supplemented with ethylene inhibitors. When leaflet, petiolule, and petiole of A. keiskei were cultured on MS medium supplemented with 2, 4-D, callus was well induced from leaflet segments at 2.0 ppm 2, 4-D. Shoot elongation of plantlets and shooting from embryogenic callus of A. keiskei were best on 2, 4-D-free medium supplemented with 2 ppm AgNO_3 or 10 ppm CoCl_2{cdot}6H_2O, but it was suppressed on the medium containing 1 ppm 2, 4-D with AgNO_3 or CoCl_2{cdot}6H_2O. Root elongation of plantlets from embryogenic callus was best on 2, 4-D-free medium supplemented with 1 ppm AgNO_3 or 5 ppm CoCl_2{cdot}6H_2O, but rooting from embryogenic callus was none on the medium containing 1 ppm 2, 4-D with AgNO_3 or CoCl_2{cdot}6H_2O. Fresh weight of plantlets from embryogenic callus was heaviest on 2, 4-D-free medium supplemented with 2 ppm of AgNO_3 or CoCl_2{cdot}6H_2O, while it was heaviest on the medium containing 1 ppm 2, 4-D with 1 ppm AgNO_3 or 2 ppm CoCl_2{cdot}6H_2O.
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KEYWORD
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Angelica keiskeiKoidz, Plant regeneration, Ethylene inhibitor, AgNO_3, CoCl_2{cdot}6H_2O
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